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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Anticardiolipin antibody,Behçet's Disease,Vascular Thrombosis
- چکیده:
- چکیده انگلیسی:
Objective: The clinical value of IgG anticardiolipin antibody in patients with Behçet's disease with or without vascular thrombosis was evaluated.
Methods: IgG isotype of anticardiolipin (aCL) antibody was assessed in 40 Behçet's disease (BD) patients with venous or arterial thrombosis, 40 BD patients without venous or arterial thrombosis and 80 healthy subjects as controls. The levels of IgG aCL were determined by an indirect ELISA method. Color Doppler Sonography, Magnetic Resonance Imaging and conventional angiography were the procedures used for other clinical evaluations.
Results: Out of 40 patients with vascular thrombosis, 20(50%) were positive for low to moderate level of IgG aCL. In patients without thrombosis 22(55%) were positive for low to moderate level of IgG aCL while in none (0%) of the healthy subjects the IgG aCL was positive, neither low nor moderate. The number of patients with headache but having a normal cerebral magnetic resonance imaging (MRI), was higher in anticardiolipin positive patients without vascular thrombosis as compared to those with vascular thrombosis, (P = 0.001). Arthritis was noticed in both patents groups. 15% of aCL positive patients without thrombosis had arthritis as compared to none in aCL negative patients without thrombosis (P = 0.02).
Conclusion: The results of this study indicate that although the frequency of IgG aCL was found to be higher in Iranian patients with BD in comparison with the previous reports, except in arthritis the observed elevated IgG aCL does not correlate with clinical disease manifestations, or vascular thrombotic complications.- انتشار مقاله: 15-05-1395
- نویسندگان: Akbar Rajaee,Mohammad Ali Nazarinia,Seyed Modjtaba Hakim,Mitra Amini,Maryam Ayatollahi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی:
Objective: To evaluate the effect of dialysis and kidney transplantation on serum levels of several tumor markers such as PSA, AFP, CA125, CA19- 9, CA15-3, CEA and to compare with normal age matched controls.
Methods: Between September of 2000 and July of 2001, the following tumor markers: PSA, AFP, CA125, CA19-9, CA15-3 and CEA were measured by ELISA Method in 29 hemodialyzed patients (group A), in 30 successfully transplanted patients (group B) and in 30 normal volunteers who did not present any clinical symptoms or signs of neoplasia.
Results: The serum level of CEA was above the cutoff limit in 6.7% of hemodialyzed patients (group A) but was in the normal range in transplanted and control groups. The level of CEA were significantly higher in hemodialyzed patients in comparison to other groups (p<0.003). Serum levels of AFP and PSA were not significantly different between the three groups (p=0.595 and p=0.545, respectively). Although serum level of CA 19-9 was elevated in 3.3% of hemodialyzed and control group the differences between the three groups were not significant (p=0.507). Serum level of CA 125 was elevated in 13.3% of group A, 13.8% of group B and 6.7% of control group (p=0.347). Serum level of CA15-3 was elevated in 13.3%, 6.9% of group A, B and control group, respectively and the differences were not significant (p=0.156).
Conclusion: Hemodialyzed and transplanted patients show a high false positive rate of CEA, CA125 and CA15-3 and may be unreliable for monitoring of malignancies in these patients while other markers evaluated (AFP, PSA and CA 19.9) appear to maintain their specificity in these situations.- انتشار مقاله: 13-05-1395
- نویسندگان: Ali Derakhshan,Masoumeh Mohkam,Abbas Ghaderi,Ghamar Hosseini Alhashemi,Mohammad Hossein Fallahzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: ELISA,Vitiligo,Rheumatoid Factors,Immunoperoxidase
- چکیده:
- چکیده انگلیسی: Background: Vitiligo is a dermatological disorder of unknown etiology with a common incidence in southern Iran. Presence of autoantibodies to melanocyte antigens suggested an autoimmune basis of the disease.
Objective: In this study, the presence of rheumatoid factor (RF) in sera and skin biopsies of vitiligo patients was investigated.
Methods: The presence of RF in sera of 35 vitiligo and 32 normal individuals was assessed by an indirect ELISA assay. In addition, the presence of IgM, IgG, and IgA immunoglobulins in the biopsy lesions of patients was also investigated by Immunoperoxidase test.
Results: IgM-RF and IgA-RF were detected in sera of 50% and 20% of patients, respectively. Five out of 35 (15%) revealed to produce both IgM and IgA rheumatoid factors. The rheumatoid factor activity of the deposited immunoglobulins at the site of lesion was confirmed by direct immunoperoxidase test.
Conclusion: The presence of rheumatoid factors as non organ-specific autoantibodies in vitiligo provides further evidence for the autoimmune etiology of the disease and its pathological importance remains to be elucidated.- انتشار مقاله: 13-05-1395
- نویسندگان: Fatemeh Vahedi Darmian,Soheila Joubeh,Mehrnoosh Doroudchi,Behnam Abdollahi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Basic Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی: Head and neck squamous cell carcinoma,matrix metalloproteinase,Extra cellular matrix
- چکیده:
- چکیده انگلیسی: Objective(s) Matrix metalloproteinases comprise a family of enzyme that is able to degrade components of extra cellular matrix. There are single nucleotide polymorphisms in the promoter regions of several genes with ability to influence cancer susceptibility. The aim of this study was to analyses association between MMP2 and MMP9 promoter polymorphisms and head and neck squamous cell carcinoma occurrence and progression. Materials and Methods A case- control study was performed including 80 head and neck squamous cell carcinoma patients and healthy controls for MMP2 and 86 head and neck squamous cell carcinoma patients and 72 healthy controls for MMP9. Blood samples were genotyped for MMP2 and MMP9 using polymerization chain reaction– restriction fragment length polymorphism method (PCR-RFLP). Statistical analysis was performed using SPSS 12.0 software. Results Our results showed that distribution of MMP2 genotype between controls and patients was significantly different (χ2= 10.3, P= 0.005). Comparison between CC genotype in HNSCC patients and controls showed that C allele modified the risk of HNSCC progression (OR= 2.6, 95% CI, 1.0046–6.729). The MMP9 genotype distribution among HNSCC patients was significantly different (χ2= 14.56, P= 0.0007). The frequency of TT genotype in HNSCC patients was different from healthy controls and was more common genotype in HNSCC cases (OR= 2.18, 95% CI, 0.7052–6.7854). Conclusion Our results suggested an association of the MMP2 and MMP9 SNP with the development of HNSCC. Also, our results showed that MMP, MMP9 genotypes and smoking were related to HNSCC progression.
- انتشار مقاله: 30-06-1394
- نویسندگان: Samaneh Hajihoseini,Mirza Khalil Bahmani,Ayyoob Khosravi,Eslam Ghezelsofla,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Biotechnology
- نوع مقاله: Journal Article
- کلمات کلیدی: Vimentin,Hybridoma,Monoclonal antibody,Pancreatic acinar cell carcinoma
- چکیده:
- چکیده انگلیسی: Background: Pancreatic carcinoma is the fourth-leading cause of cancer death in the United States and due to its late presentation, only few patients would be candidates for the curative treatment of pancreactomy. Monoclonal antibodies have brought hope to targeted therapy.
Objectives: To identify new biomarkers, a panel of monoclonal antibodies was generated against newly established cell line, Faraz-ICR from a patient with pancreatic acinar cell carcinoma.
Material and Methods: Balb/c female mice were immunized with Faraz-ICR cell line and their spleenocytes fused with SP2/0 myeloma cell line. Highly reactive hybridoma producing antibodies against Faraz-ICR was detected using ELISA, immunofluorescence staining and flow cytometry. Western blot and 2D immunoblot were utilized for further characterization of the target antibodies.
Results: Among highly reactive clones, the reactivity of 7C11 clone was assessed in comparison to other epithelial tumors. The antibody isotype was IgM that reacted with a 55 kDa protein in western blot analysis. To further characterize the target antigen, immunoproteome of the Faraz-ICR cell line was performed. By LC-MS analysis, the target of 7C11 clone was identified to be vimentin.
Conclusions: Pancreatic cancer is a highly lethal malignancy with no reliable biomarker for early detection and diagnosis. In this study, by establishing a pancreatic acinar carcinoma cell line, a panel of monoclonal antibodies was generated to identify specific or associated cancer targets. Furthermore, 7C11 mAb was introduced that can specifically recognizes vimentin as a tumor marker. This antibody may serve as a new tool for prognostic and therapeutic strategies.- انتشار مقاله: 26-10-1395
- نویسندگان: Marzie Rezaei,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Geopersia
- نوع مقاله: Journal Article
- کلمات کلیدی: Barremian-Aptian,Echinodermata,Kopeh-Dagh,Tirgan Formation,Urgonien facies type.
- چکیده: A stratigraphic section from the Tirgan Formation was selected in Arkan village nearby Bojnourd city in order to study echinoderm fauna systematically. Many specimens of echinoderms, especially toxasteridae which is associated with brachiopods were collected and studied systematically in this paper. Microscopie slides were also prepared and studied. All slides contain benthic foraminifers and calcareous algae as well as gastropod and coral fragments. Based upon diagnostic micro and macro fossils, a Barremian-Aptian age is assigned to the Tirgan Formation which suggests Urgonien facies type indicating shallow water of open marine environment The Urgonien is named after the village with this name in the east of Tarascon, France. This facies type is characteristic of shallow-water carbonate facies which was accumulated along the Tethys northern shelf from the Barremian to the late Albian. The facies consists of hard, white-gray limestones which contains Orbitolina (foraminifers) and transitional sediments – detrital or siliceous limestones (Foury, 1968).
- چکیده انگلیسی: A stratigraphic section from the Tirgan Formation was selected in Arkan village nearby Bojnourd city in order to study echinoderm fauna systematically. Many specimens of echinoderms, especially toxasteridae which is associated with brachiopods were collected and studied systematically in this paper. Microscopie slides were also prepared and studied. All slides contain benthic foraminifers and calcareous algae as well as gastropod and coral fragments. Based upon diagnostic micro and macro fossils, a Barremian-Aptian age is assigned to the Tirgan Formation which suggests Urgonien facies type indicating shallow water of open marine environment The Urgonien is named after the village with this name in the east of Tarascon, France. This facies type is characteristic of shallow-water carbonate facies which was accumulated along the Tethys northern shelf from the Barremian to the late Albian. The facies consists of hard, white-gray limestones which contains Orbitolina (foraminifers) and transitional sediments – detrital or siliceous limestones (Foury, 1968).
- انتشار مقاله: 11-10-1348
- نویسندگان: Morteza Taherpour Khalilabad,Ali Asghar Aryaei,Alireza Ashouri,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: Breast cancer,Cell line establishment,Triple-negative
- چکیده:
- چکیده انگلیسی: Breast cancer is the most common malignancy and the leading cause of cancer-related death among women
worldwide. The underlying mechanisms for breast cancer development, especially in young women, are not completely
understood. Although there are several experimental models to understand the biology of breast cancer such as
immortalized cell lines, many of these cell lines have been in culture for decades and most of them have been derived
from Caucasians or African-Americans. So, it is required to establish a new cell line derived from primary tumors and
Asian women. In this study Pari-Institute for Cancer Research (Pari-ICR) was derived from the primary breast tumor
of a 36-years old patient with invasive ductal carcinoma. We characterized the cell line by examining morphology,
expression of different markers, and functional profile. Immunocytochemistry showed that this cell line does not express
estrogen and progesterone receptors as well as human epidermal growth factor receptor 2 (HER2). Pari-ICR cell line
expresses high levels of Vimentin, Ezrin, and S100 but does not express EpCAM, Cytokeratin19, Pan-cytokeratin,
Nestin, and Desmin. Its doubling time of Pari-ICR was about 22h and was able to grow as colonies in soft agar. It
displayed a higher ability of migration and invasion in comparison with MCF-7 cell line. This breast cancer cell line
can serve as a model for understanding the molecular mechanisms of breast carcinogenesis. Moreover, it can be used
as an appropriate resource to find novel biomarkers or assess new drugs.- انتشار مقاله: 10-05-1397
- نویسندگان: Farzaneh Ghaderi,Fereshteh Mehdipour,Ahmad Hosseini,Abdolrasoul Talei,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: CXCL12,CXCR4, HNSCC, Serum
- چکیده:
- چکیده انگلیسی: Background: Squamous cell carcinoma (SCC) is the most frequent malignancy of the head and neck (HN) region.
We here evaluated associations of stromal cell derived factor-1 (SDF-1or CXCL12) and its receptor, CXCR4, with
HNSCCs. Materials and Methods: Sixty newly diagnosed HNSCC patients were enrolled in the patient group, and 28
healthy individuals in the control group. Plasma levels of CXCL12 and CXCR4 were measured using ELISA kits.
Results: There was a significant difference in mean CXCL12, but not CXCR4, plasma levels between the patient and
control groups (P=0.0001). No significant associations were found between mean plasma levels of either CXCL12 or
CXCR4 with age, gender, tumor site, tumor size, lymph-node involvement or tumor stage. Conclusion: For the first
time, our findings demonstrate a significant association between serum CXCL12 but not CXCR4 levels and HNSCCs.- انتشار مقاله: 19-02-1396
- نویسندگان: Fatemeh Lavaee,Sahar Zareh,Zahra Mojtahedi,Mahyar Malekzadeh,Bijan Khademi,Abbas Ghaderi
- مشاهده