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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Leishmania,Monoclonal antibody,gp63
- چکیده:
- چکیده انگلیسی: Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated recombinant gp63 molecules were studied by an ELISA assay. These two antibodies reacted with the crude soluble antigens prepared from 4 reference strains of Leishmania, 10 isolates from the patients, purified gp63 and recombinant gp63 molecules. However, no reaction with several non-leishmanial antigens was observed. Reaction of both antibodies with the intact recombinant gp63 and truncated molecules were compared.
Results: The results indicated that the two antibodies specifically recognize two different epitopes on the gp63 molecule.
Conclusion: Possible applications of such antibodies in searching for immunogenic epitopes are discussed.- انتشار مقاله: 15-05-1395
- نویسندگان: Ezzatallah Basiri,Sadreddin Mohseni Ardehali,Mehrnoosh Doroudchi,Fereidoun Mahboodi,Arsalan Kharazmi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Epilepsy,Adults,Autoantibodies,Sodium Valproate
- چکیده:
- چکیده انگلیسی:
Objective: To investigate possible immunological humoral correlates in newly diagnosed adult-onset generalized tonic-clonic epilepsy among Iranian patients before and after sodium valproate treatment. Patients and
Methods: 72 adult patients with newly diagnosed idiopathic generalized tonic-clonic epilepsy were recruited. Serum antinuclear antibodies (ANA), anti-cardiolipin antibodies (aCL), anti-dsDNA antibodies, total serum immunoglobulins (IgM, IgG, IgA) and C3 and C4 complements were determined before and after 12 months of therapy with sodium valproate. Similar parameters were also measured in 32 age and sex-matched healthy volunteers.
Results: Patients group had a significantly greater level of IgG class aCL (30.6% versus 12.4%, P = 0.004) and anti-dsDNA antibodies (23.9% versus 0%, P = 0.001) when compared with healthy volunteers, however, ANA titre was relatively the same in both groups. Sodium valproate significantly decreased anti-dsDNA antibodiles (P = 0.002), IgM concentrations (P = 0.034), and increased the number of ANA positive patients (P = 0.002).
Conclusion: Changes in serum level of autoantibodies in patients with new onset idiopathic generalized convulsion were found to be high. These abnormalities are associated with both seizure disorders per se and also antiepileptic drugs. We suggest that in epileptic patients with an autoimmune basis, administration of anti-epileptic drugs having modulatory effects on immune system should be considered.- انتشار مقاله: 15-05-1395
- نویسندگان: Gholamali Yousefi-Pour,Sadegh Izadi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Anticardiolipin antibody,Behçet's Disease,Vascular Thrombosis
- چکیده:
- چکیده انگلیسی:
Objective: The clinical value of IgG anticardiolipin antibody in patients with Behçet's disease with or without vascular thrombosis was evaluated.
Methods: IgG isotype of anticardiolipin (aCL) antibody was assessed in 40 Behçet's disease (BD) patients with venous or arterial thrombosis, 40 BD patients without venous or arterial thrombosis and 80 healthy subjects as controls. The levels of IgG aCL were determined by an indirect ELISA method. Color Doppler Sonography, Magnetic Resonance Imaging and conventional angiography were the procedures used for other clinical evaluations.
Results: Out of 40 patients with vascular thrombosis, 20(50%) were positive for low to moderate level of IgG aCL. In patients without thrombosis 22(55%) were positive for low to moderate level of IgG aCL while in none (0%) of the healthy subjects the IgG aCL was positive, neither low nor moderate. The number of patients with headache but having a normal cerebral magnetic resonance imaging (MRI), was higher in anticardiolipin positive patients without vascular thrombosis as compared to those with vascular thrombosis, (P = 0.001). Arthritis was noticed in both patents groups. 15% of aCL positive patients without thrombosis had arthritis as compared to none in aCL negative patients without thrombosis (P = 0.02).
Conclusion: The results of this study indicate that although the frequency of IgG aCL was found to be higher in Iranian patients with BD in comparison with the previous reports, except in arthritis the observed elevated IgG aCL does not correlate with clinical disease manifestations, or vascular thrombotic complications.- انتشار مقاله: 15-05-1395
- نویسندگان: Akbar Rajaee,Mohammad Ali Nazarinia,Seyed Modjtaba Hakim,Mitra Amini,Maryam Ayatollahi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Arthritis,systemic lupus erythematosus,Anticardiolipin antibody,Antiphospholipid syndrome,Juvenile Rheumatoid
- چکیده:
- چکیده انگلیسی: Background: Antiphospholipid antibody syndrome (APS) can either occur as a primary syndrome or associated with other autoimmune diseases such as systemic lupus erythematosus (SLE). Anticardiolipin antibody (aCL) of IgG and/or IgM isotype in blood, measured by a standardized ELISA is the most acceptable laboratory criteria. APS IgG isotype, particularly IgG2 subclass is more strongly associated with thrombosis.
Objectives: This study was done to determine the prevalence of IgG aCL and its subclasses in relation to APS symptoms, in a group of juvenile rheumatoid arthritis (JRA) and juvenile systemic lupus erythematosus (SLE) patients.
Methods: In this prospective study, 28 JRA and 16 SLE patients, aged 3-18 years, were enrolled. IgG aCL was assayed by standard aCL ELISA. IgG subclasses were also assayed by ELISA on sera with medium to high titers of aCL. ACL assay was performed on at least two occasions for each patient, over 3-6 months period of follow up.
Results: 29% (8/28) of JRA patients and 44% (7/16) of SLE patients had aCL. Six of SLE patients displayed APS related manifestations: hemolytic anemia, thrombocytopenia, arterial occlusion, valvular heart disease, livedo reticularis and pulmonary hypertension, but none of them had persistant medium or high titer of aCL. The lack of association of high titer of aCL with APS related symptoms was observed in two patients. The IgG subclasses were primarily IgG1 and IgG3.
Conclusion: The prevalence of IgG aCL in this group of pediatric SLE and JRA is not uncommon but it’s relation to clinical manifestations is not clear. IgG1 and IgG3 subclasses were not associated with thrombosis, which is in agreement with previous studies.- انتشار مقاله: 15-05-1395
- نویسندگان: Sara Kashef,Reza Amin,Maryam Ayatollahi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی:
Objective: To evaluate the effect of dialysis and kidney transplantation on serum levels of several tumor markers such as PSA, AFP, CA125, CA19- 9, CA15-3, CEA and to compare with normal age matched controls.
Methods: Between September of 2000 and July of 2001, the following tumor markers: PSA, AFP, CA125, CA19-9, CA15-3 and CEA were measured by ELISA Method in 29 hemodialyzed patients (group A), in 30 successfully transplanted patients (group B) and in 30 normal volunteers who did not present any clinical symptoms or signs of neoplasia.
Results: The serum level of CEA was above the cutoff limit in 6.7% of hemodialyzed patients (group A) but was in the normal range in transplanted and control groups. The level of CEA were significantly higher in hemodialyzed patients in comparison to other groups (p<0.003). Serum levels of AFP and PSA were not significantly different between the three groups (p=0.595 and p=0.545, respectively). Although serum level of CA 19-9 was elevated in 3.3% of hemodialyzed and control group the differences between the three groups were not significant (p=0.507). Serum level of CA 125 was elevated in 13.3% of group A, 13.8% of group B and 6.7% of control group (p=0.347). Serum level of CA15-3 was elevated in 13.3%, 6.9% of group A, B and control group, respectively and the differences were not significant (p=0.156).
Conclusion: Hemodialyzed and transplanted patients show a high false positive rate of CEA, CA125 and CA15-3 and may be unreliable for monitoring of malignancies in these patients while other markers evaluated (AFP, PSA and CA 19.9) appear to maintain their specificity in these situations.- انتشار مقاله: 13-05-1395
- نویسندگان: Ali Derakhshan,Masoumeh Mohkam,Abbas Ghaderi,Ghamar Hosseini Alhashemi,Mohammad Hossein Fallahzadeh
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: ELISA,Vitiligo,Rheumatoid Factors,Immunoperoxidase
- چکیده:
- چکیده انگلیسی: Background: Vitiligo is a dermatological disorder of unknown etiology with a common incidence in southern Iran. Presence of autoantibodies to melanocyte antigens suggested an autoimmune basis of the disease.
Objective: In this study, the presence of rheumatoid factor (RF) in sera and skin biopsies of vitiligo patients was investigated.
Methods: The presence of RF in sera of 35 vitiligo and 32 normal individuals was assessed by an indirect ELISA assay. In addition, the presence of IgM, IgG, and IgA immunoglobulins in the biopsy lesions of patients was also investigated by Immunoperoxidase test.
Results: IgM-RF and IgA-RF were detected in sera of 50% and 20% of patients, respectively. Five out of 35 (15%) revealed to produce both IgM and IgA rheumatoid factors. The rheumatoid factor activity of the deposited immunoglobulins at the site of lesion was confirmed by direct immunoperoxidase test.
Conclusion: The presence of rheumatoid factors as non organ-specific autoantibodies in vitiligo provides further evidence for the autoimmune etiology of the disease and its pathological importance remains to be elucidated.- انتشار مقاله: 13-05-1395
- نویسندگان: Fatemeh Vahedi Darmian,Soheila Joubeh,Mehrnoosh Doroudchi,Behnam Abdollahi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Polymorphism,CTLA-4,PCR-RFLP,Autoimmune,Vitiligo,Melanocyte
- چکیده:
- چکیده انگلیسی: Background: Vitiligo is an acquired skin disorder that selectively destroys melanocytes in epidermis with an unknown etiology.
Objective: To investigate the exon 1 A49G polymorphism of cytotoxic T lymphocyte antigen-4 (ctla-4) gene in vitiligo patients.
Methods: The A49G polymorphism was detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method in 101 patients and 208 normal healthy age/ethnicity matched individuals.
Results: The frequencies of heterozygote genotypes in patients and controls were found to be 42 (41.6%) of 101 and 85 (40.9%) of 208, respectively. The frequencies of homozygote A and G genotypes were 49 (48.5%) and 10 (9.9%) in 101 patients, whereas, these frequencies in 208 control individuals were 103 (49.5%) and 20 (9.6%), respectively. There was no significant difference between the genotype (P = 0.98) and allele (P = 0.86) frequencies of A49G polymorphism in patients and normal healthy individuals.
Conclusion: Our results indicate that in contrast to several immune mediated disorders, there is no association between ctla-4 A49G gene polymorphism and vitiligo.- انتشار مقاله: 15-05-1395
- نویسندگان: Mohammad Javad Fattahi,Abdul Mohammad Pezeshki,Maryam Emad,Mohammad Hosein Lohrasb,Azra Shamseddin,Abbas Ghaderi,Mehrnoosh Doroudchi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Leishmania,Monoclonal antibody,gp63
- چکیده:
- چکیده انگلیسی: Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated recombinant gp63 molecules were studied by an ELISA assay. These two antibodies reacted with the crude soluble antigens prepared from 4 reference strains of Leishmania, 10 isolates from the patients, purified gp63 and recombinant gp63 molecules. However, no reaction with several non-leishmanial antigens was observed. Reaction of both antibodies with the intact recombinant gp63 and truncated molecules were compared.
Results: The results indicated that the two antibodies specifically recognize two different epitopes on the gp63 molecule.
Conclusion: Possible applications of such antibodies in searching for immunogenic epitopes are discussed.- انتشار مقاله: 15-05-1395
- نویسندگان: Ezzatallah Basiri,Sadreddin Mohseni Ardehali,Mehrnoosh Doroudchi,Fereidoun Mahboodi,Arsalan Kharazmi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Arthritis,systemic lupus erythematosus,Anticardiolipin antibody,Antiphospholipid syndrome,Juvenile Rheumatoid
- چکیده:
- چکیده انگلیسی: Background: Antiphospholipid antibody syndrome (APS) can either occur as a primary syndrome or associated with other autoimmune diseases such as systemic lupus erythematosus (SLE). Anticardiolipin antibody (aCL) of IgG and/or IgM isotype in blood, measured by a standardized ELISA is the most acceptable laboratory criteria. APS IgG isotype, particularly IgG2 subclass is more strongly associated with thrombosis.
Objectives: This study was done to determine the prevalence of IgG aCL and its subclasses in relation to APS symptoms, in a group of juvenile rheumatoid arthritis (JRA) and juvenile systemic lupus erythematosus (SLE) patients.
Methods: In this prospective study, 28 JRA and 16 SLE patients, aged 3-18 years, were enrolled. IgG aCL was assayed by standard aCL ELISA. IgG subclasses were also assayed by ELISA on sera with medium to high titers of aCL. ACL assay was performed on at least two occasions for each patient, over 3-6 months period of follow up.
Results: 29% (8/28) of JRA patients and 44% (7/16) of SLE patients had aCL. Six of SLE patients displayed APS related manifestations: hemolytic anemia, thrombocytopenia, arterial occlusion, valvular heart disease, livedo reticularis and pulmonary hypertension, but none of them had persistant medium or high titer of aCL. The lack of association of high titer of aCL with APS related symptoms was observed in two patients. The IgG subclasses were primarily IgG1 and IgG3.
Conclusion: The prevalence of IgG aCL in this group of pediatric SLE and JRA is not uncommon but it’s relation to clinical manifestations is not clear. IgG1 and IgG3 subclasses were not associated with thrombosis, which is in agreement with previous studies.- انتشار مقاله: 15-05-1395
- نویسندگان: Sara Kashef,Reza Amin,Maryam Ayatollahi,Abbas Ghaderi
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Immunology
- نوع مقاله: Journal Article
- کلمات کلیدی: Epilepsy,Adults,Autoantibodies,Sodium Valproate
- چکیده:
- چکیده انگلیسی:
Objective: To investigate possible immunological humoral correlates in newly diagnosed adult-onset generalized tonic-clonic epilepsy among Iranian patients before and after sodium valproate treatment. Patients and
Methods: 72 adult patients with newly diagnosed idiopathic generalized tonic-clonic epilepsy were recruited. Serum antinuclear antibodies (ANA), anti-cardiolipin antibodies (aCL), anti-dsDNA antibodies, total serum immunoglobulins (IgM, IgG, IgA) and C3 and C4 complements were determined before and after 12 months of therapy with sodium valproate. Similar parameters were also measured in 32 age and sex-matched healthy volunteers.
Results: Patients group had a significantly greater level of IgG class aCL (30.6% versus 12.4%, P = 0.004) and anti-dsDNA antibodies (23.9% versus 0%, P = 0.001) when compared with healthy volunteers, however, ANA titre was relatively the same in both groups. Sodium valproate significantly decreased anti-dsDNA antibodiles (P = 0.002), IgM concentrations (P = 0.034), and increased the number of ANA positive patients (P = 0.002).
Conclusion: Changes in serum level of autoantibodies in patients with new onset idiopathic generalized convulsion were found to be high. These abnormalities are associated with both seizure disorders per se and also antiepileptic drugs. We suggest that in epileptic patients with an autoimmune basis, administration of anti-epileptic drugs having modulatory effects on immune system should be considered.- انتشار مقاله: 15-05-1395
- نویسندگان: Gholamali Yousefi-Pour,Sadegh Izadi,Abbas Ghaderi
- مشاهده