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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Background: Most research on autonomic dysfunction of diabetes mellitus is conducted on ganglions innervating gastrointestinal (GI) tract and there are limited works focusing on cervical sympathetic ganglia. The effects of diabetes mellitus (DM) on the neurons of superior cervical sympathetic ganglion (SCSG) are investigated by stereological methods. Material and Methods: Female rats (n=72) randomly divided into DM (blood glucose =400-600 mg/dl) and control (n=36) groups. Rats were sacrificed at 4-, 8- and 12-weeks of induction of DM (65 mg/kg streptozotocin, ip). The same procedure followed chronologically in control group. SCSG of both groups were removed, fixed, and embedded in cylindrical blocks. Isotropic uniform random sections obtained and stained. The mean particle volume (according to the method of volume-weighted mean particle estimation) of the perikarya and nuclei of ganglion cells (neurons) were estimated using the point-sample intercepts method. Results: There was no significant difference between the mean perikaryal and nuclear volumes of DM and control rats after 4-, 8- and 12-weeks. There was, however, a significant increase in the mean volume of perikarya and nuclei of the neuronal cells of DM rats at 8- and 12-weeks diabetes as compared with those of 4-weeks. Conclusion: The mean volume of SCSG and their nuclei were not significantly reduced after 4-, 8-and 12-weeks in DM rats and these cells continued their normal growth.Iran J Med Sci 2005; 30(1): 24-27. Keywords ● Diabetes mellitus ● Stereology ● Autonomic ganglia
- انتشار مقاله: 09-06-1394
- نویسندگان: A. Noorafshan,M. Azizi,E. Aliabadi,S. Karbalay-Doust
- مشاهده
- جایگاه : پژوهشی
- مجله: Iranian Journal of Medical Sciences
- نوع مقاله: Journal Article
- کلمات کلیدی:
- چکیده:
- چکیده انگلیسی: Background: Pyelonephritis can lead to renal scar. Objective: To evaluate the effects of betamethasone in preventing renal scarring in rat model of pyelonephritis. Methods: Sixty three female Sprague-Dawley rats were divided into seven groups. Group A was the control. Mannose-sensitive E coli was directly inoculated into the left kidney exposed under general anesthesia in groups C–G. Group B received normal saline. Two days after bacterial inoculation, groups C, E and F received gentamicin for 10 days. Betamethasone was injected for three days to animals in groups of D, E (2 days after bacterial inoculation) and F (5 days after bacterial inoculation). Group G received no treatment. Eight weeks after bacterial inoculation, animals were sacrificed and the volume (amount) of renal scar was determined using the stereological techniques. Results: Changes in the weight and volume of the kidneys were not statistically significant. No scar was detected in group A, but all other groups including group B with intrarenal injections showed scarring. The volume density and absolute volume of the scar in groups C–G were significantly more than group B (p<0.001), whereas they did not differ significantly from each other. Conclusion: Betamethasone and/or gentamicin, when used two days after induction of pyelonephritis were not effective in preventing renal scar.
- انتشار مقاله: 07-07-1394
- نویسندگان: M.H. Fallahzadeh,A. Noorafshan,N. Tanideh,H.R. Badeli,A. Bazargani
- مشاهده