در هنگام جستجو کلمه در قسمت عنوان میتوانید کلمات مورد جستجو را با کاراکتر (-) جدا کنید.
کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Chemical Engineering(IJChE)
- نوع مقاله: Journal Article
- کلمات کلیدی: fractional calculus,Shrinkage,Finite hollow cylinder,Diffusion phenomena,Anomalous diffusion models
- چکیده:
- چکیده انگلیسی: Water and solid effective diffusivities and shrinkage were correlated for finite hollow cylinder-shaped apple samples during the candying operation in the osmotic solution. Experiments were conducted in the sucrose solution as an osmotic agent at different temperatures (i.e., 40, 50, and 60 °C) and at a constant concentration of 55 °Brix. The effective diffusivities of water and solid were calculated by fitting the water loss and solid uptake experimental data to Fick’s second law and fractional calculus method, considering the shrinkage of the samples during the candying process. The obtained results exhibited that the volume of the apples reduced linearly by increasing the water loss. For above conditions of the candying process, water effective diffusivities with Fick second law were determined in the range of 3.7×10−10 m2/s–8.73×10−10 m2/s, and those with fractional calculus method were in the range of 2.75×10−10 m2/s–6.98×10−10 m2/s. The results indicated that the coefficient of determination for the fractional calculus method was more than the coefficient of determination for the Fick model. The value of the empirical parameter α for the Non-Fickian diffusion model was always higher than unity, meaning that the dehydration process had a super-diffusive mechanism.
- انتشار مقاله: 16-04-1399
- نویسندگان: A. Etemadi,R. Alizadeh,M. Sirousazar
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: PCR,Real-time PCR,Brucella abortus,identification
- چکیده:
- چکیده انگلیسی: Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This study aimed to present a novel conventional and real-time polymerase chain reaction (PCR) assay for the identification of Brucella abortus strains. Regarding this, two primers (bru ab2) were designed based on the unique loci encoding autotransporter-associated beta strand repeat-containing protein (ID:YP00113760). A total of 56 Brucella strains (e.g., reference, vaccinal, and field isolates) and Yersinia enterocolitica, as a non-Brucella isolate, were evaluated in conventional and real-time PCR systems. The results of the study indicated that 0.4 ng and 400 FG of genomic DNA of B. abortus strains can be detected by conventional and real-time PCR, respectively. The primers, bru ab2, were suitable for both PCR methods. Both methods were specific for the detection of all strains of the bacterium; however, real-time PCR assay was 1000-fold more sensitive than the conventional PCR method. Therefore, this new detection system could be a suitable selective modified method for the accurate identification of all B. abortus strains.
- انتشار مقاله: 07-06-1397
- نویسندگان: S. Alamian,T. Zahraei Salehi,K. Aghaiypour Kolyani,M. Esmaelizad,A. Etemadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Antigenic seed,Brucella abortus S99,Molecular test,Validation
- چکیده:
- چکیده انگلیسی: Brucellosis is a zoonotic infection that is associated with fever in humans and abortion in animals. The agent of this disease is a facultative intracellular gram-negative coccobacillus called Brucella. There are six classic species, including B. abortus, B. melitensis, B. suis, B. canis, B. neotomae, and B. ovis. In recent years, four new species have been reported, including Brucella ceti, B. microti, B. pinnipedialis, and B. inopinata. Human disease causes hygienic and economic losses, including inactivity of workforces in the community and high cost of treatment. The disease also causes catastrophic losses in the livestock industry. There is no effective vaccine against human brucellosis. Hence, attempts to prevent human infection with Brucella are focused on preventative measures, including control of infection in livestock, which lead to a reduction in its incidence in humans. The common methods for diagnosis of this disease are serologic methods including Rose Bengal, Wright -2 ME and the ring test. B. abortus strain S99 is used to produce these diagnostic antigens. The production of these antigens requires the presence of a well-characterized seed with full identity. The aim of this work was confirmation of the identity of B. abortus S99 by phage typing, AMOS and multiplex PCR techniques. Therefore, it is essential to carry out the identification of the strains used as seed for the production of the brucellosis diagnostic antigens. In this project, B. abortus strain 99 was supplied by the bacterial collection of the Brucellosis Department of Razi Vaccine and Serum Research Institute. Then, the main aim of the present study was the confirmation of the seed identity by doing the tests through the standard phage typing method, AMOS PCR and multiplex PCR (Brucladder) methods. Results were in support of the identity of the studied strain, and the molecular methods could also be used as the sensitive approaches for validation of antigenic seed.
- انتشار مقاله: 16-07-1397
- نویسندگان: S. Alamian,M. Dadar,S. Soleimani,A.M. Behrozikhah,A. Etemadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Antigenic seed,Brucella abortus S99,Molecular test,Validation
- چکیده:
- چکیده انگلیسی: Brucellosis is a zoonotic infection that is associated with fever in humans and abortion in animals. The agent of this disease is a facultative intracellular gram-negative coccobacillus called Brucella. There are six classic species, including B. abortus, B. melitensis, B. suis, B. canis, B. neotomae, and B. ovis. In recent years, four new species have been reported, including Brucella ceti, B. microti, B. pinnipedialis, and B. inopinata. Human disease causes hygienic and economic losses, including inactivity of workforces in the community and high cost of treatment. The disease also causes catastrophic losses in the livestock industry. There is no effective vaccine against human brucellosis. Hence, attempts to prevent human infection with Brucella are focused on preventative measures, including control of infection in livestock, which lead to a reduction in its incidence in humans. The common methods for diagnosis of this disease are serologic methods including Rose Bengal, Wright -2 ME and the ring test. B. abortus strain S99 is used to produce these diagnostic antigens. The production of these antigens requires the presence of a well-characterized seed with full identity. The aim of this work was confirmation of the identity of B. abortus S99 by phage typing, AMOS and multiplex PCR techniques. Therefore, it is essential to carry out the identification of the strains used as seed for the production of the brucellosis diagnostic antigens. In this project, B. abortus strain 99 was supplied by the bacterial collection of the Brucellosis Department of Razi Vaccine and Serum Research Institute. Then, the main aim of the present study was the confirmation of the seed identity by doing the tests through the standard phage typing method, AMOS PCR and multiplex PCR (Brucladder) methods. Results were in support of the identity of the studied strain, and the molecular methods could also be used as the sensitive approaches for validation of antigenic seed.
- انتشار مقاله: 16-07-1397
- نویسندگان: S. Alamian,M. Dadar,S. Soleimani,A.M. Behrozikhah,A. Etemadi
- مشاهده