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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Iranian Journal of Veterinary Research
- نوع مقاله: Journal Article
- کلمات کلیدی: Biological control,Sciomyzidae,Sepedon,Lymnea
- چکیده:
- چکیده انگلیسی: The present work was carried out to investigate first, the ecology of the Sepedon flies as well as species
of Lymnea snails and secondly, the biological effects of Sepedon fly larvae living on Lymnea. In this regard,
the life cycle, death rate and compatibility of Lymnea species, as well as the life cycle of Sepedon flies in
breeding cage and the activity of the new larvae in the same ecological condition were investigated. The
results showed that Lymnea truncatula needs more sophisticated ecological and feeding conditions than other species of the snails. Lymnea stagnalis was found sensitive to temperature variation and this may lead to gradual or sudden death. Lymnea pregra and Lymnea palustris could well adapt with laboratory conditions.
The killing effect (P<0.001) of the third-stage larvae on snails (3 to 4 weeks) was more than the effect of
first- and second-stages larvae. This effect was more in a container with 2 cm water depth than a container
with 5 cm depth.- انتشار مقاله: 10-01-1385
- نویسندگان: Gh. R. Motamedi,A. H. Dalimi Asl,M. A. Akhavizadegan,R. Pilehchian Langroodi,M. Abdigoudarzi,M. Mohammadi
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Leishmania infantum,Recombinant protein,Dog,Visceral leishmaniasis,PQ10,Serodiagnosis
- چکیده:
- چکیده انگلیسی: Mediterranean visceral leishmaniasis is a zoonotic disease caused by Leishmania infantum and transmitted by the bites of infected female sand flies. Iran is one of the endemic areas of this disease. Dogs and canines are the major reservoir hosts of Leishmania infantum in the new and old world, including Iran. If visceral leishmaniasis is left untreated, it may result in a 90% mortality rate. The identification and elimination of infected dogs are efficient ways to control this disease. The diagnostic methods used to identify these animals cannot yield 100% detection. Therefore, in the present study, we used a multiepitope recombinant protein (PQ10) to distinguish between symptomatic and asymptomatic infections caused by Leishmania infantum in animal reservoirs (dogs). The coding sequence of PQ10 recombinant protein was sub-cloned in pET28 expression vector and was commercially synthesized by GENERAY Biotechnology, China. In the following process, sequencing was performed with plasmid-specific primers and followed by the expression, optimization of expression. The purified recombinant protein was confirmed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot. The efficacy of recombinant PQ10 protein was evaluated by an indirect enzyme-linked immunosorbent assay (ELISA) test using 150 dog sera (25 symptomatic positive sera, 25 asymptomatic positive sera, 80 negative sera, and 20 sera of dogs with other infectious diseases). Direct agglutination test (DAT) as the standard method was used to compare and determine specificity and sensitivity. The results indicated that the sensitivity and specificity of ELISA using infected dog sera were 94% and 86%, respectively. Positive and negative predictive values for dog sera were reported as 87.03% and 93.47%, respectively. This protein was able to identify 92% of asymptomatic dogs with visceral leishmaniasis. The results showed that the recombinant protein PQ10 is able to identify positive cases of canine visceral leishmaniasis, especially asymptomatic cases.
- انتشار مقاله: 13-03-1398
- نویسندگان: F. Jameie,A. Dalimi,M. Pirestani,M. Mohebali
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Iran,Dog,Neospora caninum,Fox,Indirect Fluorescent Antibody Test (IFAT),Nested- polymerase chain reaction (PCR),Sanandaj
- چکیده:
- چکیده انگلیسی: Neospora caninum, a protozoan parasite, causes abortions in cattle, as well as neurological disorders and reproductive problems in dogs. This study aimed to investigate the serological and the molecular prevalence of N.caninum among foxes and dogs using indirect fluorescent antibody test (IFAT) and nested-polymerase chain reaction (PCR). For this purpose, 288 and 95 both fecal and serum samples of dogs and foxes were collected, respectively, from around industrial and traditional dairy flocks in different parts of Sanandaj, Kurdistan Province, Iran, from 2013 to 2015. The sera were examined using IFAT, and fecal samples were microscopically assessed for detecting Neospora oocyst and by nested-PCR. The findings revealed that N.caninum seroprevalence were 4.86% and 4.21% in dogs and foxes, respectively. In addition, no Neospora oocysts were found microscopically and by PCR. Since this study is the first serological and molecular investigation of N.caninum among both dogs and foxes in Sanandaj, the findings of indicated that stray dogs is a main source of N.caninum infection in dairy farms in Sanandaj, Iran.
- انتشار مقاله: 04-04-1397
- نویسندگان: Gh. Adhami,A. Dalimi,N. Hoghoooghi-Rad,Sh. Fakour
- مشاهده
- جایگاه : پژوهشی
- مجله: Archives of Razi Institute
- نوع مقاله: Journal Article
- کلمات کلیدی: Tehran,PCR,Dog,Hepatozoon canis
- چکیده:
- چکیده انگلیسی: Hepatozoonosis is a protozoal disease caused by various species of Hepatozoon. This parasite is transmitted from tick; the main vector of Hepatozoon canis is usually the brown dog tick (Rhipicephalus sanguineus). However, several species of ticks are disposed as the alternative vectors. Dogs are usually infected by eating the tick or a part of the tick organ infected by the mature oocysts containing infectious sporozoite. In the current study, a total of 145 blood samples were collected from the cephalic vein of pet, stray, and shelter dogs in Tehran. To conduct this study, first thin blood smears were prepared from all the samples and stained with the Giemsa method. Then, after extraction of DNA from the blood samples, in order to trace Hepatozoon canis, the 18S rRNA gene segment of the parasite was amplified using polymerase chain reaction (PCR). To confirm the PCR-positive results, five randomly selected PCR-positive samples were sequenced. According to the results, through direct observation of microscopic slides, no infection of H. canis parasite was observed, but according to the PCR results, 32 out of the 145 blood samples were found to be infected by H. canis. In this study, infection to H. canis in older dogs was higher than in young dogs, and more male dogs were found to be infected by the parasite compared to female dogs; but no significant difference was observed in this regard (P > 0.05). Moreover, stray dogs showed a significantly higher rate of infection, compared to the pet and shelter ones (P < 0.05).
- انتشار مقاله: 01-02-1396
- نویسندگان: R. Soltani,A. Dalimi
- مشاهده