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کاربرد نوع شرط:
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: head and neck cancer,Polymorphism,DNA Methylation,Folate
- چکیده:
- چکیده انگلیسی: Background: Folate is essential for DNA synthesis, repair, and methylation. Polymorphisms in genes associated with folate metabolism may alter these processes and, consequently, modulate cancer development. Aim: We aimed to assess DNMT3B -149C/T (rs2424913), DNMT3B -283T/C (rs6087990), DNMT3B -579G/T (rs2424909), DHFR 19-pb ins/del (rs70991108), SHMT1 1420C/T (rs1979277), and TYMS 28-bp tandem repeat (rs34743033) polymorphisms with risk of head and neck cancer. Methods: A case-control study was conducted in 1,086 Brazilian individuals. Real-time and conventional polymerase chain reactions-PCR were performed for genotyping the polymorphisms. Results: The single nucleotide polymorphism (SNP), DNMT3B -283T/C, revealed a higher risk of head and neck squamous cell carcinoma (HNSCC) when compared with the C group in the codominant (p < 0.001), dominant (p <0.001), and overdominant (p= 0.001) models for T/C and C/C genotypes. DNMT3B -149C/T and DNMT3B -579G/T revealed no association between groups in any model. The DHFR 19-pb ins/del polymorphism protected against HNSCC development compared to the C group by the codominant (p < 0.001), dominant (p < 0.001), and overdominant (p < 0.001) models. In the TYMS, the 3R/3R genotype had a protective effect against HNSCC development compared with the C group by the recessive models (p= 0.009). In contrast, SHMT1 1420 C/T presented no association between the HNSCC and C groups. DHFR 19-pb ins/del polymorphisms protected against oral cavity cancer (p= 0.003), and only TYMS-28 3R/3R decreased the risk of tumor progression (p= 0.023). In the Kaplan–Meier curve, an association was found between DHFR ins/ins and TYMS -28 3R carriers with respect to relapse-free time; further, DNMT3B -579 T and TYMS-28 2R/2R carriers had longer survival times. Conclusion: DNMT3B -283T/C is associated with higher risk, whereas DHFR 19-pb ins/del and TYMS 28 3R/3R protect against head and neck cancer. We also highlighted the association of TYMS 3R/3R genotype carriers with relapse-free cancer protection and survival time.
- انتشار مقاله: 27-04-1399
- نویسندگان: Tialfi Bergamin De Castro,Gabriela Helena Rodrigues-Fleming,Juliana Garcia De Oliveira-Cucolo,Jéssika Nunes Gomes Da Silva,Fabia Pigatti Silva,Luiz Sérgio Raposo,José Victor Maniglia,Erika Cristina Pavarino,Lidia Maria Rebolho Batista Arantes,Ana Lívia Silva Galbiatti-Dias,Eny Maria Goloni Bertollo
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: oxidative stress,Biomarker,carcinoma squamous cell,oral cavity,carcinogens
- چکیده:
- چکیده انگلیسی: Background: Alteration in the biotransformation of exogenous compounds can result in production of reactive
oxygen species (ROS), which can predispose cells to malignant transformation in the head and neck. This study aimed
to evaluate the expression of genes involved in antioxidant metabolism in the oral squamous cell carcinoma (OSCC).
Methods: The expression of eighty-four genes was evaluated in OSCC and non-tumor tissues by quantitative real-time
polymerase chain reaction using the TaqMan Gene Expression Array. The biological mechanisms related to
the differentially expressed genes were investigated using Gene – NCBI, KEGG, UNIPROT and REACTOME databases.
Results: Twenty-one genes encoding enzymes involved in antioxidant metabolism were differentially expressed in
the OSCC case. Four genes (ATOX1, PRDX4, PRNP, and SOD2) were up-regulated, and seventeen (ALOX12, CAT,
CSDE1, DHCR24, DUOX1, DUOX2, EPHX2, GLRX2, GPX3, GSR, GSTZ1, MGST3, PRDX1, OXR1, OXSR1,
SOD1, and SOD3) were down-regulated. We identified 14 possible novel biomarkers for OSCC. The differentially
expressed genes appeared related to important biological processes involved in carcinogenesis, such as inflammation,
angiogenesis, apoptosis, genomic instability, invasion, survival, and cell proliferation. Conclusions: Our study
identified novel biomarkers which might warrant further investigation regarding OSCC pathogenesis since the altered
expression in the genes can modulate biological processes related to oxidative stress and predispose cells to malignant
transformation in the oral cavity.- انتشار مقاله: 29-09-1396
- نویسندگان: Nayara Fernandes Pedro,Joice Matos Biselli,José Victor Maniglia,Dalisio Neto,Erika Cristina Pavarino,Eny Maria Goloni-Bertollo,Patricia Matos Biselli-Chicote
- مشاهده
- جایگاه : پژوهشی
- مجله: Asian Pacific Journal of Cancer Prevention
- نوع مقاله: Journal Article
- کلمات کلیدی: head and neck cancer,VEGFA,alternative splicing,pathologic angiogenesis,Gene expression
- چکیده:
- چکیده انگلیسی:
Background: Overexpression of proangiogenic vascular endothelial growth factor A family VEGFAxxx is associated with tumor growth and metastasis. The role of the alternatively spliced antiangiogenic family VEGFAxxxb is poorly investigated in head and neck squamous cell carcinomas (HNSCCs). The antiangiogenic isoform binds to bevacizumab and its expression level could influence the treatment response and progression-free survival. In this study, the relative expression of VEGFAxxx and VEGFA165b isoforms and splicing regulatory factors genes was investigated in a series of HNSCCs. Methods: VEGFAxxx, VEGFA165b, SRSF6, SRSF5, SRSF1 and SRPK1 gene expression was quantified by quantitative real time PCR in 53 tissue samples obtained by surgery from HNSCC patients. Protein expression was evaluated by immunohistochemistry. Results: VEGFAxxx and VEGFA165b were overexpressed in HNSCCs. Elevated protein expression was also confirmed. However, VEGFA isoforms demonstrated differential expression according to anatomical sites. VEGFAxxx was overexpressed in pharyngeal tumors while the VEGFA165b isoform was up-regulated in oral tumors. The VEGFA165b isoform was also positively correlated with expression of the splicing regulatory genes SRSF1, SRSF6 and SRSF5. Conclusions: We concluded that VEGFAxxx and VEGFA165b isoforms are overexpressed in HNSCCs and the splicing regulatory factors SRSF1, SRSF6, SRSF5 and SRPK1 may contribute to alternative splicing of the VEGFA gene. The findings for the differential expression of the antiangiogenic isoform in HNSCCs could facilitate effective therapeutic strategies for the management of these tumors.- انتشار مقاله: 28-01-1396
- نویسندگان: Patrícia Matos Biselli-Chicote,Joice Biselli,Bianca Cunha,Rodrigo Castro,José Victor Maniglia,Dalísio De Santi Neto,Eloiza Helena Tajara,José Franscisco De Góis Filho,Erica Erina Fukuyama,Érika Cristina Pavarino,Eny Maria Goloni-Bertollo
- مشاهده