The Synergistic Cytotoxic Effect of Laser-Irradiated Gold Nanoparticles and Sorafenib Against the Growth of a Human Hepatocellular Carcinoma Cell Line

The Synergistic Cytotoxic Effect of Laser-Irradiated Gold Nanoparticles and Sorafenib Against the Growth of a Human Hepatocellular Carcinoma Cell Line

  • عنوان فارسی : The Synergistic Cytotoxic Effect of Laser-Irradiated Gold Nanoparticles and Sorafenib Against the Growth of a Human Hepatocellular Carcinoma Cell Line
  • عنوان انگلیسی : The Synergistic Cytotoxic Effect of Laser-Irradiated Gold Nanoparticles and Sorafenib Against the Growth of a Human Hepatocellular Carcinoma Cell Line
  • مجله : Asian Pacific Journal of Cancer Prevention
  • issn(مجله) : 1513-7368
  • نوع مقاله: Journal Article
  • زبان: انگلیسی
  • نوع انتشار: مقاله چاپ شده
  • انتشار مقاله: 10-11-1397
  • شماره(No): 11
  • جایگاه : پژوهشی
  • دوره: 20
  • شماره صفحه: 3369,3376
  • تعداد نویسندگان: 5
  • doI: 10.31557/APJCP.2019.20.11.3369
  • مقاله مستخرج از:
  • نویسندگان: Haidy M Ebrahim , Mahmoud N El-Rouby , Mona E Morsy , Mahmoud M Said , Magda K Ezz ,
  • چکیده:
  • چکیده انگلیسی: Gold nanoparticles are the most promising candidate in cancer treatment due to their physiochemical properties
    and increased use in photothermal therapy (PTT). In the present study, spherical gold nanoparticles (AuNPs) were
    synthesized using citrate reduction method. The particles were then characterized using UV-VIS spectroscopy and
    transmission electron microscope. A hepatocellular carcinoma cell line (HepG2) was incubated with sorafenib and/or
    non-irradiated or laser-irradiated AuNPs for 48 hrs. The cytotoxic effect of different treatment modalities was determined
    using MTT assay. Furthermore, apoptosis was determined by flow cytometry using annexin V/propidium iodide, as
    well as estimating the level of caspases. Results showed that AuNPs and sorafenib reduced HepG2 cell viability, and
    the cytotoxicity was associated with increased release of LDH in the culture medium. The recorded cytotoxicity was
    attributed to enhanced apoptosis as revealed by increased cellular caspases (3, 8 and 9), that was further confirmed by
    flow cytometry. The most notable cytotoxic effect was recorded when combining sorafenib with laser-irradiated AuNPs.
    In conclusion, a synergistic cytotoxic effect was observed between sorafenib and laser-irradiated AuNPs against the
    growth of HepG2, suggesting the potential substitution of large toxic doses of sorafenib by lower doses in combination
    with photothermal therapy.
The Synergistic Cytotoxic Effect of Laser-Irradiated Gold Nanoparticles and Sorafenib Against the Growth of a Human Hepatocellular Carcinoma Cell Line

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