Phylogeny and Classification of Human Papillomavirus (HPV)16 and HPV18 Variants Based on E6 and L1 genes in Tunisian Women with Cervical Lesions

Phylogeny and Classification of Human Papillomavirus (HPV)16 and HPV18 Variants Based on E6 and L1 genes in Tunisian Women with Cervical Lesions

  • عنوان فارسی : Phylogeny and Classification of Human Papillomavirus (HPV)16 and HPV18 Variants Based on E6 and L1 genes in Tunisian Women with Cervical Lesions
  • عنوان انگلیسی : Phylogeny and Classification of Human Papillomavirus (HPV)16 and HPV18 Variants Based on E6 and L1 genes in Tunisian Women with Cervical Lesions
  • مجله : Asian Pacific Journal of Cancer Prevention
  • issn(مجله) : 1513-7368
  • نوع مقاله: Journal Article
  • زبان: انگلیسی
  • نوع انتشار: مقاله چاپ شده
  • انتشار مقاله: 25-06-1396
  • شماره(No): 12
  • جایگاه : پژوهشی
  • دوره: 19
  • شماره صفحه: 3361,3366
  • تعداد نویسندگان: 11
  • doI: 10.31557/APJCP.2018.19.12.3361
  • مقاله مستخرج از:
  • نویسندگان: Mouna Jendoubi-Ferchichi , Latifa Satouri , Fatma Ghoul , Monia Malek-Mellouli , Abdel Moneem Derbal , Mohamed Kamel Makni , Hedi Reziga , Ali Baba , Mohamed Zili , Michel Segondy , Ridha Khelifa ,
  • چکیده:
  • چکیده انگلیسی: Background: High-risk human papillomavirus (HPV) types are the main etiological factors for cervical cancer.
    HPV16 and HPV18 are generally the most common forms associated with development of high-grade cervical lesions.
    This study was undertaken to identify intratypic variants of HPV16 and HPV18 among women with cervical lesions
    in Tunisia. Materials and Methods: DNA was extracted from cervical samples collected from 49 women. using a
    PureLinkTM Genomic DNA mini Kit (Invitrogen). E6 and L1 open reading frames (ORF) were amplified by PCR
    and viral DNA amplicons were subjected to automated sequencing using Big Dye Terminators technology (Applied
    Biosystems). The obtained sequences were analyzed using an appropriate software program to allow phylogenetic
    trees to be generated. Results: HPV16 and HPV18 were detected in 15 and 5 cases, respectively. HPV16 E6 sequences
    clustered with the European German lineage (A2) whereas one isolate diverged differently in the L1 region and
    clustered with the African sub-lineage (B1). HPV 18 E6 sequences clustered with the European sub-lineage (A1)
    but L1 sequences clustered as a new clade which diverged from A1-A5. Conclusions: Our results suggest that the
    distribution of HPV16 and HPV18 sequences in women with cervical lesions in Tunisia is mainly related to European
    epidemiological conditions and point to the presence of recombinant HPV forms.
Phylogeny and Classification of Human Papillomavirus (HPV)16 and HPV18 Variants Based on E6 and L1 genes in Tunisian Women with Cervical Lesions

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