چکیده انگلیسی:
Background: Hydrocarbons degradation is principally achieved by microorganisms in natural environments. The extent of hydrocarbons biodegradation is mainly conditioned by environmental factors and its success depends on the optimal condition for the crude oil degrading isolates. Objectives: The aims of the current study was to isolate and identify crude oil degrading bacterium from surface sediments of Qeshm Island, Iran and to evaluate the efficiency of statistically-based experimental design for the optimization of crude oil degradation performed by the isolated strain. Materials and Methods: Crude oil degrading bacteria were isolated by serial dilutions of bacterial consortium. In order to optimize crude oil biodegradation by isolated strain, Plackett-Burman experimental design was used to evaluate nine factors affecting crude oil biodegradation in twelve experimental trials. To observe the best yield in crude oil biodegradation, factor which had higher effects were considered for the next stage in the biodegradation optimization process using the Taguchi experimental design. Results: A gram-negative bacterium strain signed as KK1- strain (with 98% homology with Marinobacter litoralis) was isolated from enrichment consortium. Among the various variables screened using Plackett-Burman experimental design, pH, temperature, salinity and NH4Cl were determined as the most significant factors and considered for the next stage in the biodegradation optimization process using the Taguchi experimental design. Theoretically, the optimum degradation conditions were determined as: pH = 8, temperature = 35˚C, salinity = 30 ppt and NH4Cl = 1 g.L-1. The validity of the predicted optimized condition was tested by conducting experiment considering the predicted criteria. Biodegradation efficiency of 58.32±5.57% was achieved under suggested condition which was significantly higher than the primary condition (35%). Conclusions: Indigenous bacteria from surface sediments of Qeshm Island were found to be able to degrade crude oil. Our results showed that a combination of the Plackett-Burman and the Taguchi experimental design may be successfully used to find the optimal amounts of those factors for crude oil biodegradation.
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