Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil

عنوان فارسی : Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
عنوان انگلیسی : Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
مجله : Iranian Journal of Biotechnology
issn(مجله) : 1728-3043
نوع مقاله: Journal Article
زبان: انگلیسی
نوع انتشار: مقاله چاپ شده
انتشار مقاله: 21-02-1394
شماره(No): 2
جایگاه : پژوهشی
دوره: 5
شماره صفحه: 110,113
تعداد نویسندگان: 5
doI: 9426
مقاله مستخرج از:
نویسندگان: Hamidreza Falahatpishe , Mahmoud Jalali , Naser Badami , Nadia Mardani , Kianoush Khosravi-Darani ,
چکیده:
چکیده انگلیسی: This research has focused on isolation and characterization of a strain of Bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at pHs ranging from 8 to 11 and temperatures of 20 to 50ºC. Also the impact of different carbon and nitrogen sources were investigated. The yield and fold of enzyme purification was 24% and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline protease produced by Bacillus sp. 2 - 5 showed the most caseinolytic activity (without any gelatinolytic activity) at pH>10

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